DOI: https://doi.org/10.22141/2309-8147.7.2.2019.169682

Experimental justification of using low doses of atropine sulfate in the stabilization of morphological structure of sclera and lamina cribrosa

P.A. Bezditko, A.O. Gulida

Abstract


Background. The prevalence of ophthalmopathology significantly affects the health of the population, causes a decrease in the capacity for work and significantly deteriorates the quality of life. Using atropine sulfate in low doses can stabilize myopia progression and myopic changes in the posterior pole of the eye. The purpose was to study the morphological structures of the eye after the application of low doses of atropine sulfate in experimental animals. Materials and methods. The study involved 19 Chinchilla rabbits (38 eyes). They were 5 months old and weighed 2.5 kg. Animals were observed for 6 months, after which they were withdrawn from the experiment. During the study, the atropine sulfate instillations were applied at a concentration of 0.5% once a day. The control group included 11 rabbits (22 eyes) of the same weight and age. Materials for morphological study were enuclea­ted eyeballs of animals from all groups fixed in 10% formalin solution for 72 hours. In each group, 5–8 randomly selected fields of view were studied. Results. The data of morphological examination showed that in the region of the posterior pole of the eyeball in the study group animals compared to the control group, there was a thickening of the fibrous tunic of eyeball, the thickness of the sclera significantly increased, as well as the thickness of the lamina cribrosa and the wall of it pores, and a significant increase in the intensity of type I and III collagen glow. The morphofunctional state of the retina does not change. Conclusions. Using 0.5% solution of atropine sulfate helps to strength the fibrous membrane of the eye wall, sclera and lamina cribrosa and its pores, with no changes in the morphofunctional state of the receptor apparatus of the eye.


Keywords


morphological examination; myopia; lamina cribrosa; atropine sulfate

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