DOI: https://doi.org/10.22141/2309-8147.6.2.2018.172237

ANTIOXIDANT SYSTEM IN THE NERVOUS TISSUES OF THE EYE IN THE OPHTHALMOHYPERTENSION IN ANIMALS WITH STREPTOZOTOCIN DIABETES

V. R. Yurevych

Abstract


Introduction. Relevant is the search for drugs to normalize metabolic processes in the tissues of the eye in diabetes and ophthalmohypertension.
Herewith, the effectiveness of drug therapy is determined by its pathogenetic orientation. Therefore, clarifi cation of the pathogenesis of these pathologies remains consistent.
Рurpose. Examine the state of the antioxidant system in the tissues of the eye in the simulation hypertension (experimental glaucoma) in animals with streptozotocin diabetes.
Methods. Studies were conducted on 32 rabbits. The experimental animals were divided into four groups: the fi rst group – control group (8 rabbits), the second – the experimental group of animals with diabetes in terms of hypertension (10 rabbits), the third – the experimental group of animals with diabetes (7 rabbits), the fourth – the experimental group animals with hypertension (7 rabbits). The activity of catalase, superoxide dismutase and glutathione peroxidase was identifi ed in isolated tissues of the retina and optic nerve tissue.
Results. Analyzing the study results of the activity of antioxidant enzymes in the neuronal tissues of the eye in the modeling hypertension in animals with streptozotocin diabetes, a signifi cant reduction in the capacity of neutralization of reactive oxygen species was traced.
Conclusions. 1. It was found out that the development of ophthalmohypertension in animals with diabetes leads to a more pronounced reduction in the capacity of neutralization of reactive oxygen species in the eye tissues compared with diabetes and ophthalmohypertension in separate groups of animals. Superoxide dismutase and catalase in the retina and optic nerve with concomitant development of hypertension and diabetes was signifi cantly lower by 16.5% and 17.9% respectively compared with diabetic conditions.
2. It was found out that, when combined with the effects of ocular hypertension and hyperglycemia, the rate of neutralization of lipid hydroperoxides in the neural elements of the visual analyzer was signifi cantly reduced compared with the normal and hypertensive animals. Glutathione peroxidase activity was reduced under these conditions by 43.2% and 32.8% respectively.


Keywords


ocular hypertension, diabetes, retina and optic nerve, superoxide dismutase, catalase, glutathione peroxidase

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