DOI: https://doi.org/10.22141/2309-8147.6.1.2018.172269

EFFECT OF DIABETIC RETINOPATHY PROGRESSION FACTORS ON THE CONTENT OF MARKER OF ADHESIVE ENDOTHELIAL DYSFUNCTION VCAM-1 IN THE BLOOD AT TYPE 2 DIABETES MELLITUS AS A CLUSTER OF METABOLIC SYNDROME

L. Yu. Pylypenko, V. M. Serdiuk

Abstract


Background. One of the key roles in the development of diabetic retinopathy (DRP) is capillary occlusion. As a possible mechanism of this phenomenon is the increase of the leukocytes adhesion to the endothelium due to high expression of adhesive molecules in the microcirculatory space of the retina.

Purpose. To study the content of the vascular cell adhesion molecule VCAM-1 in the blood at different stages of the DRP.
Material and methods. The research was carried out with 64 patients (95 eyes) with at type 2 diabetes mellitus (T2DM), metabolic syndrome (MS) and DRP (age 61.55±2.37 years old, length of diabetes 11.23±2.11 years, level of HbA1C 9.89±0.78 %, BMI 34.55±3.75 kg/m2), who were divided into 3 groups depending on the stage of DRP. Exclusion criteria for this study were: type 1 diabetics, mental disorders, age less than 18 years, severe comorbidities such as congestive heart failure, liver disease, malignancy, infl ammatory process, pregnancy, or any factor affecting body weight such as hypothyroidism, corticosteroids (Cushing’s syndrome), or contraceptives. Written informed consent was obtained from all subjects prior to the inclusion into the study. The ANOVA and regression analysis were used as statistical analysis.
Results. The highest value of the VCAM-1 level of blood was in the nonproliferative stage of DRP (3.45±0.39 pg/ml). Regardless of the stage of the DRP, a signifi cant direct linear relationship was found between VCAM-1 and fi brinogen of the blood (r=0.59, R2=35.1 %, p=0.01). The tendency in the direct dependence of the VCAM-1 content and the duration of T2DM was revealed (r=0.25, R2=6.5 %, p=0.05).
Conclusions. Possible participation of VCAM-1 together with fi brinogen in the formation of leukostasis and capillary occlusion, especially in the nonproliferative stage of DRP was traced.


Keywords


diabetic retinopathy, VCAM-1, type 2 diabetes mellitus, metabolic syndrome

References


Сердюк В. Н. Морфометрические и биохимические кластеры метаболического синдрома у пациентов с сахарным диабетом 2-го типа на разных стадиях диабетической ретинопатии / В. Н. Сердюк, В. А. Ищенко // Міжнародний ендокриноло-гічний журнал. – 2016. – Т. 7, № 7. – С. 69–74.

Adamiec-Mroczek J. Assessment of selected adhesion molecule and proinfl ammatory cytokine levels in the vitreous body of patients with type 2 diabetes-role of the infl ammatory-immune process in the pathogenesis of proliferative diabetic retinopathy / J. Adamiec-Mroczek, J. Ofi cjalska-Mlynczak // Graefes Arch Clin Exp Ophthalmol. – 2008. – Vol. 246. – P. 1665–1670.

Aiello L. P. Diabetic retinopathy / L. P. Aiello, T. W. Gardner, G. L. King [et al.] // Diabetes Care. – 1998. – Vol. 21. – P. 143–156.

Antonetti D. A. Diabetic Retinopathy: Seeing Beyond Glucose-Induced Microvascular Disease / D. A. Antonetti, A. J. Barber, S. K. Bronson [et al.] // Diabetes. – 2006. – Vol. 55. – Р. 2401–2411.

Barile G. R. Soluble cellular adhesion molecules in proliferative vitreoretinopathy and proliferative diabetic retinopathy / G. R. Barile, S. S. Chang, L. S. Park [et al.] // Curr Eye Res. – 1999. – Vol. 19. – P. 219–227.

Cybulsky M. I. A major role for VCAM-1, but not ICAM-1, in early atherosclerosis / M. I. Cybulsky, K. Iiyama, H. Li [et al.] // J. Clin. Invest. – 2001. – Vol. 107, N 10. – P. 1255–1262.

Executive summary of the third report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults (Adult Treatment PanelIII) // JAMA. – 2001. – Vol. 285. – Р. 2486–2497.

Garner A. Histopathology of diabetic retinopathy in man / A. Garner // Eye. – 1993. – Vol. 7 (2). – P. 250–253.

Gho Y. S. Angiogenic activity of human soluble intercellular adhesion molecule-1 / Y. S. Gho, H. K. Kleinman, G. Sosne // Cancer Res. – 1999. – Vol. 59. – P. 5128–5132.

Goldberg R. B. Cytokine and cytokine-like infl ammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications / R. B. Goldberg // J Clin Endocrinol Metab. – 2009. – Vol. 94. – P. 3171–3182.

Grundy S. M. Diagnosis and Management of the Metabolic Syndrome / S. M. Grundy, J. I. Cleeman, S. R. Daniels [et al.] // Circulation. – 2005. – Vol. 112. – Р. 2735–2752.

Gustavsson C. Vascular Cellular Adhesion Molecule-1 (VCAM-1) Expression in Mice Retinal Vessels Is Affected by Both Hyperglycemia and Hyperlipi demia / C. Gustavsson, C-D. Agardh, A. V. Zetterqvist [et al.] // PLoS ONE. – 2010. – Vol. 5 (9). – e12699.

Gustavsson C.TNF-alpha is an independent serum marker for proliferative retinopathy in type 1 diabetic patients / C. Gustavsson, E. Agardh, B. Bengtsson, C. D. Agardh // J Diabetes Complications. – 2008. – Vol. 22. – P. 309–316.

Hatchell D. L. Neutrophils plus capillaries in acute experimental retinal ischemia / D. L. Hatchell, C. A. Wilson, P. Saloupis // Microvasc Res. – 1994. – Vol. 47. – P. 344–354.

Hughes J. M. Vascular leucocyte adhesion molecules unaltered in the human retina in diabetes / J. M. Hughes, A. Brink, A. N. Witmer [et al.] // Br J Ophthalmol. – 2004. Vol. 88. – P. 566–572.

Inzucchi S. Management of Hyperglycemia in Type 2 Diabetes, 2015: A Patient-Centered Approach: Update to a Position Statement of the American Diabetes Association and the European Association for the Study of Diabetes / S. Inzucchi, R. M. Bergenstal, J. B. Buse [et al.] // Diabetes Care. – 2015. – Vol. 38 (1). – Р. 140–149.

Khan Z. A. Cellular signaling and potential new treatment targets in diabetic retinopathy / Z. A. Khan, S. Chakrabarti // Exp Diabetes Res. – 2007. – Vol. 2007. – P. 31867.

Kohner E. M. Screening for diabetic retinopathy in Europe: A fi eld handbook / E. M. Kohner, M. Porta. – Boehringer Mannheim, Italy. – 1992.

Konno S. Retinal blood fl ow changes in type I diabetes. A long-term follow-up study / S. Konno, G. T. Feke, A. Yoshida [et al.] // Invest Ophthalmol Vis Sci. – 1996. – Vol. 37. – P. 1140–1148.

Kurose I. Molecular determinants of reperfusion-induced leukocyte adhesion and vascular protein leakage / I. Kurose, D. C. Anderson, M. Miyasaka [et al.] // Circ Res. – 1994. – Vol. 74. – P. 336–343.

Larson R. S. Structure and function of leukocyte integrins / R. S. Larson, T. A. Springer // Immunological Reviews. – 1990. – Vol. 114. – P. 181–217.

Libby P. Infl ammation in atherosclerosis. / P. Libby // Nature. – 2002. – Vol. 420. – P. 868–874.

Limb G. A. Vascular adhesion molecules in vitreous from eyes with proliferative diabetic retinopathy / G. A. Limb, J. Hickman-Casey, R. D. Hollifi eld, A. H. Chignell // Invest Ophthalmol Vis Sci. – 1999. – Vol. 40. – P. 2453–2457.

Matsumoto K. Comparison of serum concentrations of soluble adhesion molecules in diabetic microangiopathy and macroangiopathy / K. Matsumoto, Y. Sera, Y. Ueki [et al.] // Diabet Med. – 2002. – Vol. 19. – P. 822–826.

Nguyen T. T. Infl ammatory, Hemostatic and Other Novel Biomarkers for Diabetic Retinopathy: The Multi-Ethnic Study of Atherosclerosis / T. T. Nguyen, E. Alibrahim, F. M. A. Islam [et al.] // Diabetes Care. – 2009. – Vol. 32. – P. 1704–1709.

Nowak M. Blood serum levels of vascular cell adhesion molecule (sVCAM-1), intercellular adhesion molecule (sICAM-1) and endothelial leucocyte adhesion molecule-1 (ELAM-1) in diabetic retinopathy / M. Nowak, T. Wielkoszynski, B. Marek [et al.] // Clin Exp Med. – 2008. – Vol. 8. – P. 159–164.

Sagel J. Increased platelet aggregation in early diabetes mellitus / J. Sagel, J. A. Colwell, L. Crook [et al.] // Ann Intern Med. – 1975. – Vol. 82. – P. 733–738.

Schlingemann R. O. Increased expression of endothelial antigen PAL-E in human diabetic retinopathy correlates with microvascular leakage / R. O. Schlingemann, P. Hofman, G. F. Vrensen [et al.] // Diabetologia. – 1999. – Vol. 42. – P. 596–602.

Takami S. High concentration of glucose induces the expression of intercellular adhesion molecule-1 in human umbilical vein endothelial cells / S. Takami, S. Yamashita, S. Kihara [et al.] // Atherosclerosis. – 1998. – Vol. 138 (1). – P. 35–41.

Use of Glycated Haemoglobin (HbA1c) in the Diagnosis of Diabetes Mellitus. Abbreviated Report of a WHO Consultation (WHO/NMH/CHP/CPM/11.1) / World Health Organization. – Geneva: WHO. – 2011. – 25 p.

van Buul J. D. Endothelial signaling by Ig-like cell adhesion molecules / J. D. van Buul, E. Kanters, P. L. Hordijk // Arterioscler Thromb Vasc Biol. – 2007. – Vol. 27. – P. 1870–1876.

Videm V. Soluble ICAM-1 and VCAM-1 as markers of endothelial activation / V. Videm, M. Albrigtsen // Scand J Immunol. – 2008. – Vol. 67(5). – P. 523–531.

Yan Z. CTRP3 is a novel biomarker for diabetic retinopathy and inhibits HGHL-induced VCAM-1 expression in an AMPK-dependent manner / Z. Yan, J. Zhao, L. Gan [et al.] // PLoS ONE. – 2017. – Vol. 12(6). – e0178253.

Zohlinhöfer D. Adhesion of monocyte very late antigen-4 to endothelial vascular cell adhesion molecule-1 induces interleukin-1beta-dependent expression of interleukin-6 in endothelial cells / D. Zohlinhöfer, K. Brand, K. Schipek [et al.] // Arterioscler. Thromb. Vasc. Biol. – 2000. – Vol. 20, N 2. – P. 353–359.


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