DOI: https://doi.org/10.22141/2309-8147.5.3.2017.172347

FEATURES OF BLOOD CELLS’ FATTY ACIDS CONTENT IN PATIENTS WITH DIABETIC RETINOPATHY

O. V. Petrenko, L. V. Natrus, К. К. Tavartkiladze

Abstract


The problem of developing effective regimens for the treatment of diabetes retinopathy (DR) continues to be relevant and includes the study of hemorheological disorders in type 2 diabetes. The metabolism of fatty acids (FA) has a signifi cant effect on the structure and physical properties of cell membranes (LC).
The goal is to compare the FA composition in the blood cells of patients with DR, and to determine the most informative model for studying the structural features of the body cells during lipid metabolism disorders.
Materials and methods. Blood plasma, leukocytes, platelet-rich plasma and erythrocytes were investigated in 16 patients of the ophthalmological department (the ratio of women and men 8/8), whose average age was 56.9±9.2 years and who had been diagnosed with proliferative stage DR (one or both eyes).
The average duration of type 2 diabetes in this group was 16.6±3.7 years, the blood glucose level at the study moment was 11.55±1.09 mmol/l. The indices with regard to age and sex of healthy donors were studied for comparison; the level of glucose of venous blood in them was 4.57±1.9 mmol/l. The FA composition was studied by gas chromatography.
Patients were selected with an error of deviation of the FA content in substrates no more than ±10 %. They made up a group of patients with DR for this study.
Results and discussion. Analysis of the FA content and spectrum showed a signifi cant difference in erythrocytes and platelets in patients with DR compared with a group of healthy donors. Calculations of the Spearman rank correlation coeffi cient found a high correlation of erythrocyte and healthy donor indices r=0.97 (p<0.001).
The FA concentration and content in the patients with metabolic shifts plasma is constantly changing, so that the study of the FA spectrum in plasma complicates the interpretation of the data. The study of FA in cell membranes is more appropriate. We measured the FA content in healthy donor’s different blood cells and determined the most informative environment for the FA metabolism analysis.
The methodological features of the substrate taking showed that the cell membrane which maximally isolated from the plasma can be obtained only in erythrocytes. We found that a decrease in the arachidonic FA in erythrocytes and platelets is one of the most signifi cant signs of impaired metabolism of FA in patients with DR.
Arachidonic acid is found in large numbers in platelets and, it performs one of the most important functions of platelets when they are activated. The study of the processes and mechanisms of changing the structure of the membrane, accompanied by fl uctuations of the arachidonic FA is impractical to conduct on platelets. Mix of the pathogenetic mechanisms can infl uence the interpretation of the results.
Conclusion. We believe that the erythrocyte membranes FA study is the most methodologically correct in investigation of the LC metabolism while comparing all blood cells.


Keywords


erythrocytes, platelets, fatty acids, plasma, diabetic retinopathy

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