DOI: https://doi.org/10.22141/2309-8147.5.2.2017.172453

RISK FACTORS OF DISTURBANCE OF RETINAL MICROCIRCULATION IN PATIENTS WITH TYPE II DIABETES MELLITUS

A. S. Hudz, M. L. Maksimtsyv

Abstract


The aim of the work was to study the risk factors for the disturbance of microcirculation of the retina in patients with type II diabetes mellitus. The study included 38 patients (38 eyes) with type II diabetes without clinically visible changes on the fundus. Ophthalmologic examination was performed before the beginning of treatment and included: best corrected visual acuity, tonometry, gonioscopy, biomicroscopy, ophthalmoscopy, optical coherence tomography.
Platelets (Tc) were isolated by centrifugation from the peripheral blood of patients. To activate Tc, subthreshold concentrations of agonists were used, which in the control group caused aggregation not exceeding 10 %. Evaluation of aggregation was carried out by spectrophotometric method using the Chrono-Log Aggregator (USA). The results of the study showed that in case of a stable background collagen level, the sensitivity of α2-adrenoreceptors Tc provides an increase in the proaggregant state of Tc with an increase in the concentration of catecholamines in the incubation mixture. A similar increase in Tc reactivity was registered in case of a stable background level of adrenaline and an increase in the concentration of collagen. The sequential introduction of adrenaline and platelet activating factor (PAF) into the Tc suspension at subthreshold concentrations was accompanied by an increase in aggregation that exceeded the isolated effect of each agonist (p <0.001). The fact of the summation of the effects of the two agonists confi rms the possibility of a joint action of adrenaline and collagen, and / or epinephrine and PAP in the activation of Tc for type II diabetes. Thus, activation of the sympathoadrenal system, endothelial dysfunction and infl ammation may be risk factors for increasing the proaggregant status of Tc and disturbing the microcirculation of the retina in patients with type II diabetes.


Keywords


diabetes mellitus type II, microcirculation of the retina, functional state of thrombocytes, diabetic retinopathy

References


Баринов Э. Ф. Тромбоциты / Э. Ф. Баринов, О. Н. Сулаева, А. М. Гнилорыбов. – Донецк, 2012. – 324 с.

Arthur J. F. Exacerbation of glycoprotein VI-dependent platelet responses in a rhesus monkey model of Type 1 diabetes / J. F. Arthur, Y. Shen, Y. Chen [et al.] // J. Diabetes Res. – 2013. – Vol. 2013. –Р. 370 – 212.

Cepeda-Nieto A. C. High prevalence of diabetic retinopathy and lack of association with integrin α2 gene polymorphisms in patients with type 2 diabetes from Northeastern Mexico / A. C. Cepeda-Nieto, M. T. Esquivel-Contreras, F. Duran-Iñiguez [et al.] // Exp Ther Med.– 2015. – Vol. 10, № 2. – Р. 435–444.

Cryer P. E. Hypoglycemia-associated autonomic failure in diabetes / P. E. Cryer // Handb Clin Neurol. – 2013. – Vol. 117. – Р. 295–307.

Güemes M. What is a normal blood glucose? /M. Güemes, S. A. Rahman, K. Hussain // Arch Dis Child. – Vol. 101, № 6. – Р.569–574.

Hulsmans M. MicroRNA-containing microvesicles regulating infl ammation in association with atherosclerotic disease / M. Hulsmans, P. Holvoet // Cardiovasc Res. – 2013. – Vol. 100, № 1. – P. 7–18.

Jiang Y. Beta-adrenergic receptor agonist decreases VEGF levels through altered eNOS and PKC signaling in diabetic retina / Y. Jiang, Q. Zhang, J.J.Steinle //Growth Factors. – 2015. – Vol. 33, №3. – Р. 192–199.

LaGamma E. F. Partial blockade of nicotinic acetylcholine receptors improves the counterregulatory response to hypoglycemia in recurrently hypoglycemic rats / E. F. LaGamma, N. Kirtok, O. Chan [et al.] // Am J Physiol Endocrinol Metab. – 2014. – Vol. 307, № 7. – Р. 580–588.

Martín-Cordero L. The interleukin-6 and noradrenaline mediated infl ammation-stress feedback mechanism is dysregulated in metabolic syndrome: effect of exercise / L. Martín-Cordero, J. J. García, M. D. Hinchado [et al.] // Cardiovasc Diabetol. – 2011. – Vol. 10. –Р. 42.

Martín-Cordero L. Noradrenaline-mediated inhibition of infl ammatory cytokines is altered in macrophages from obese Zucker rats: effect of habitual exe rcise / L. Martín-Cordero, J. J. García, E. Ortega [et al.] // Endocr Metab Immune Disord Drug Targets. – 2013. – Vol. 13, № 3. – Р. 234–239.

McDonald M. W. The glucoregulatory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus / M. W. McDonald, M. R. Murray, K. N. Grise [et al.] // Appl Physiol Nutr Metab. – 2016 . – Vol. 41, № 6. – Р. 631–639.

Moore S. F. Loss of the insulin receptor in murine megakaryocytes/platelets causes thrombocytosis and alterations in IGF signalling / S. F. Moore, C. M. Williams, E. Brown [et al.] // Cardiovasc Res. – 2015. – Vol. 107, № 1. – Р. 9–19.

Shim S. H. Association Between Platelet Function and Disc Hemorrhage in Patients With Normal-Tension Glaucoma: A Prospective Cross-Sectional Study / S. H. Shim, J. M. Kim, H. Y. Woo [et al.] // Am J Ophthalmol. –2015. – Vol. 160, № 6 – Р. 1191–1199.

Soma P. Flow cytometric analysis of platelets type 2 diabetes mellitus reveals ‘angry’ platelets / P. Soma, A. C. Swanepoel, J. N. Plooy [et al.] // Cardiovasc Diabetol. – 2016. – Vol. 15, № 1 – Р. 52.

Straznicky N. E. Arterial norepinephrine concentration is inversely and independently associated with insulin clearance in obese individuals with metabolic syndrome / N. E. Straznicky, M. T. Grima, E. A. Lambert [et al.] // J Clin Endocrinol Metab. – 2015. – Vol. 100, № 4. – Р. 1544–1550.

Tang W. H. Glucose and collagen regulate human platelet activity through aldose reductase induction of thromboxane / W. H. Tang, J. Stitham, S. Gleim [et al.] // J Clin Invest. – 2011. – Vol. 121, № 11. – Р. 4462–4476.

Yasunari E. Repetitive hypoglycemia increases circulating adrenaline level with resultant worsening of intimal thickening after vascular injury in male GotoKakizaki rat carotid artery / E. Yasunari, T. Mita, Y. Osonoi [et al.] // Endocrinology. – 2014. – Vol. 155, № 6 – Р. 2244–2253.


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

 

© "Publishing House "Zaslavsky", 1997-2019

 

   Seo анализ сайта